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For KEAP1 mutant analysis, 1.6 g of pGL4.37, 0.2 g of KEAP1 or GFP expression vector, and 0.2 g of pRL-SV40 were used for the transfection of a single well

with 100 g TNP-Ficoll FITC (Biosearch Technologies F-1300F) and 30 min later tissues were analyzed with flow cytometry (splenocytes were stained with surface markers for the detection of FoB and MZB, with the addition of biotinylated anti-TNP (BD Pharmingen 554055) + Streptavidin-APC (Biolegend 405207 for TNP detection) or snap-frozen for immunofluorescence microscopy in OCT (Sakura 4583)

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Co-evolution of Mycobacterium tuberculosis and Homo sapiens